Automated Ribotyping, a Rapid Typing Method for Analysis of Erysipelothrix spp. Strains

Automated ribotyping and antibiotic resistance determining of Bacillus spp from conjunctiva of diabetic patients

Objective(s): We aimed to characterize the phenotype and genotype of Bacillus spp isolated from diabetic patients’ eyes, by studying the drug sensitivity patterns with a disc-diffusion method. Materials and Methods: Fifty eyes of 25 patients with type II diabetes mellitus, with at least 10 years of diabetes history, were included in the study. We analyzed the eyes for the presence of Bacillus ...

Development of a multiplex PCR-based rapid typing method for enterohemorrhagic Escherichia coli O157 strains.

Enterohemorrhagic Escherichia coli O157 (EHEC O157) is a food-borne pathogen that has raised worldwide public health concern. The development of simple and rapid strain-typing methods is crucial for the rapid detection and surveillance of EHEC O157 outbreaks. In the present study, we developed a multiplex PCR-based strain-typing method for EHEC O157, which is based on the variability in genomic...

PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens

Comparison of rapid, automated ribotyping and DNA macrorestriction analysis of Burkholderia pseudomallei.

An automated ribotyping device (RiboPrinter) was used to determine the ribotypes of a collection of Burkholderia pseudomallei isolates. In a preliminary evaluation with the restriction enzymes BamHI and EcoRI, the protocol with EcoRI was more discriminating. The reproducibilities of the ribotypes obtained with EcoRI (EcoRI ribotypes) were determined by testing three levels of bacterial loads. T...

بررسی تنوع ژنوتیپی سویه‌های بالینی سالمونلا انتریکا سروتایپ اینفنتیس به روش ریبوتایپینگ

Background and Objective: Salmonella spp. are enteric pathogens with a worldwide distribution comprising a large number of serovars characterized by different hosts and distribution. Among Salmonella spp., the number of infections and diseases caused by the serotype Salmonella enterica serovar Infantis started to increase significantly in the last decade. The aim of this study was to investigat...